Tech. Docs

  • Viability PCR - Virus detection

    Viable PCR combines the use of photo-reactive reagents with a high affinity for DNA/RNA with a photo-chemical reaction. The nature of the reagents precludes it to pass through virus capsid. For this reason the DNA/RNA from virus with undamaged capsid will be free of photo blockage. After the treatment of microbial aqueous suspension with our reagents combined with a photo-activation step, only DNA/RNA from intact virus will be detected by molecular procedures

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  • Viability PCR - Legionella detection

    Monitoring of viable Legionellae levels in water samples is possible by the means of Viability PCR. Nowadays in a few hours it’s possible to perform a more complete evaluation of the real sanitary risk while avoiding the current approach drawbacks:

    Culture needs more than 5 days to show a first result with a final assay runtime of 10 days (according ISO 11731)

    Conventional qPCR detects DNA from total Legionellae (dead and live) and for this reasons positive results can provide a very biased interpretation of real risk.

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  • Viability PCR - Food Microbiology

    Viable real time PCR has been used as an accurate reliable simple method to detect and quantify microorganisms involved in the food production process. The technique has been applied in the determination of wine yeasts [1], Escherichia coli O157:H7 [2],Campylobacter jejuni [3], Zygosaccharomyces bailii [4], thermophilic Bacillus species [5], and probiotic bacteria [6]

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  • Viability PCR - Environmental Microbiology

    Viable real time PCR has been used as an accurate reliable simple method to detect and quantify viable microorganism in environmental samples. Some of the microorganisms detected by using this technique are Cryptosporidium [1], Bacteroides fragilis [2], Enterococcus faecalis [3], Bacteroides thetaiotaomicron [3], fungal species [4], and Legionella pneumophila [5] from others.

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  • Viability PCR - Clinical Microbiology

    Real time PCR and Terminal Restriction Fragment Length Polymorphism (T-RFLP) have been combined with Propidium monoazide, to identify viable bacteria in clinical samples. The techniques have been applied in the determination of Pseudomonas aeruginosa, Burkholderia cepacia, Escherichia coli, Staphylococcus aureus, and Mycobacterium tuberculosis.

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  • Tuberculosis Note

    Viability PCR combines the use of photoreactive reagents with a high affinity for DNA with a photochemical reaction. The nature of the reagents precludes it to pass through cell membranes. For this reason the DNA from cells with undamaged membrane will be free of photo blockage. After the treatment of microbial aqueous suspension with our reagents combined with a photoactivation step, only DNA from live microorganisms will be detected by molecular procedures

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  • Beverages and Spoilers Note

    Monitoring of spoilers levels in brewage samples is possible by the means of Viability PCR. Nowadays in a few hours it’s possible to perform a more complete evaluation of the real spoilage risk while avoiding the current approach drawbacks:

    Often, culture needs, several days to show a first result with a final assay runtime, in some cases about 10 days.

    Conventional qPCR detects DNA from total spoiler (dead and live) and for this reasons positive results can provide a very biased interpretation of real risk.

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