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Dark Box Note
During all the steps previous to DNA neutralization by the means of photo-activation it is necessary to protect the reaction solution from light, in order to avoid collateral reactions and reagent loss. Additionally, according to reagent nature, microorganisms and sample types, it is also very important to ensure temperature stability during the incubation time. With the appropriate temperature the reagent diffusion through cell we obtain: the membrane of dead microorganism could be optimized and minimizing the reagents access to live microorganism.
The dark box system combines these characteristics and is therefore the best solution for handling your samples during viability PCR process.