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Viability PCR combines the use of photo-reactive reagents with a high affinity for DNA with a photo-chemical reaction. The nature of the reagents precludes it to pass through cell membranes. For this reason the DNA from cells with undamaged membrane will be free of photo blockage. After the treatment of microbial aqueous suspension with our reagents combined with a photo-activation step, only DNA from live microorganisms will be detected by molecular procedures: PCR, qPCR, Flow Cytometry, Fluorescence Mycroscopy, LAMP.