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PEMAX™ Reagent, a new step forward in the detection of live cells by PCR
The PEMAX™ Reagent is a double dye technology developed by GenIUL, S.L. (Patent pending), in order to overcome the current limitations of viability PCR procedures. Nowadays, the paradigm is based in the cell membrane integrity of living cells as a unique differential factor from dead cells. However, as appointed in first reports, some disinfection procedures can induce cells death without compromising membrane integrity .
This new reagent, combined with the appropriate reaction buffer, extends the concept of viability PCR to cells with intact cell membrane structure but also with capability to actively maintain bacterial homeostasis, as a result of active metabolism .