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Reaction Buffers Note
One of the critical steps in all vPCR workflows is sample and reagent incubation in the dark. During this treatment it is very important to maximize reagent diffusion trough dead cells, spores, cysts and microbial aggregates. On the other hand, this procedure is also necessary to prevent viable cell damage, for this reason ionic strength, pH, nutrient level and aerobic/anaerobic balance need to be under control. GenIUL introduces three different reaction buffers: Standard, Standard Plus and Anaerobic, optimizing this procedure for different applications.