How to get the best air sampling

Indoor Air quality is a cause of adverse health effects in building occupants and a big problem for the quality of pharmaceutical, food, or cosmetics products. To control air quality, the legislations recommend carrying out air sampling through different collector methods which transfer the microorganism of this air to a surface of nutrient media (agar).

 It is fundamental to know the percentage of microorganisms recovered to select the best air sampler. To evaluate this factor, consider the impact speed and the radius of the intake nozzles and technology used to impact the airborne particles to the Petri plate. For example, small nozzles distributed in spin tend to increase impact efficiency.

In addition, connecting two air samplers and duplicate simultaneous testing is an optimal procedure to sample at the same time fungus and bacteria according to different legislation. These benefit from the use of Spin Air samplers to assess airborne bacterial and fungal bioburden.

The new standard UNE-EN 17141:2021

According to the new standard UNE-EN 17141:2021 that has recently come into effect replacing ISO 14698 Parts 1 and 2 from 2003 (the previous standard) establishes the sampling rate of 100 L/min.

Depending on the degree of contamination of the room, the sampling time can be adjusted between 5 and 10 minutes. UNE-EN 17141:202, recommends a volume between 22 and 2000 L. Therefore, through an air sampler with a 100 L/min speed, if it is used for 10 minutes, we will collect up to 1000 L.

Petri plate agar to plate microorganisms

The Petri plate agar surface to plate microorganisms is essential to guarantee an exact counting of the viable colonies. The physical collection efficiency is based on the physical features of the sampling device, such as flow rate, orifice shape, and size or the number of orifices.

The regulations EN- 17141 and the USP U.S. Pharmacopeia (USP) chapter <797> discuss and describe the microbiological growth medium and non-selective media such as tryptic soy agar with polysorbate and lecithin (TRApl) for bacteria and malt extract agar (MEA), or Destrosa Sabourad (SDA) for fungi.

Therefore, the best air sampling practice should consider carrying carry out two independents but simultaneous air sampling, one with a TRApl’s plate and the other with an MEA’s plate.


Spin Air for microbial air contamination monitoring

Microbial air contamination monitoring is a must in facilities with special air cleanliness needs. IUL’s Spin Air samplers grant users a compact, portable, simple solution to air sampling. Accuracy and precision-enhancing Spin technology enable Spin Air samplers to outperform the accuracy of other air sampling systems.

IUL’s spin technology enhances air sampling accuracy, precision, and resolution even when sampling in mid/highly contaminated environments (useful for GMP Grade C and D cleanrooms and regular environment sampling).

This sampling technology uses 100% of the Petri plate agar surface to plate microorganisms (other air samplers only use 5% of the plate), improving the statistical significance greatly and avoiding the colony count correction tables.

With the Spin Air, it is possible to choose between 100 L/min (90 mm plate) and 60 L/m (Rodac plate) to have the necessary volume specified in the current legislation. This speed is calculated according to the number of holes and their diameter. Through these specifications, a medium-sized microorganism impact at a speed of 10.8 m/s, so that is trapped on the surface but does not get stressed.

Spin Air and other samplers: a comparison

If the Spin Air is compared with other high-flow single-stage impaction-based air samplers for bacteria quantification, the spin technology becomes the most efficient for bacteria recollection. Studies have shown that Spin Air is the most precise single-stage inertial impactor, which recovers a higher number of colony-forming units (CFUs) making Spin Air the best option for indoor-air bioaerosol concentration measurements.

In addition, Spin Air makes the quantification of airborne microorganisms quicker since the positive-hole correction procedure is not necessary due to the Spin Air sampling head rotation mechanism. While Spin Air shows the highest number of CFU/m3, the error and coefficient of variation (CV) between measures are the lowest.

In contrast, other devices have a higher CV, and studies point out that this could be for unequal flow distribution, especially in those devices with two sampling heads and one aspiration pump. This problem is solved with the Spin Air, which has one main head and can use an attached sampler with its aspiration pump.

    What makes IUL’s Spin Air the best air sampling?

    Nowadays, different companies have commercialized several inertial impaction samplers, and high-flow portable samplers with an integrated sampling pump have become very popular. We can find three single-stage inertial impaction samplers that are the most used for microbiological sampling and, between them, there is the IUL’S Spin Air.

    The portable high-flow single-stage impaction-based air sampler for bacteria sampling Spin Air gives the highest concentration with the lowest data dispersion and therefore it is the most precise, accurate, and reproducible, even when sampling in mid or highly contaminated environments.

    Spin Air presents a header interchangeable and available with different materials available to be sterilized in the autoclave. What is more, the Spin Air sampler allows you to comply with USP 797 & 1116 regulations and the UNE-EN 17141 and transmit data to your LIMS with a simple connection.

    Pharmaceutical, medical device, food, and cosmetic manufacturers, as well as healthcare facilities, can benefit from the IUL’s Spin Air samplers to assess airborne bacterial and fungal bioburden.

    More reproducibility and accuracy

    Spin Air sampling head rotation mechanism and holes geometry get an efficient sampling, using the 100% of the Petri plate agar surface to plate microorganisms. The IUL’S Spin Air used without rotation (0 rpm) a correction on the results must be applied as other single-stage impaction-based air samplers for colony quantifications. The adjust considers the probability that one microorganism travels across the same hole that a previous one. This correction is not important at low counts but becomes dramatically higher when there are more than 150 CFUs per plate.

    As a result, the Spin Air technology enhances air sampling accuracy, precision, and resolution even when sampling in mid or highly-contaminated environments (useful for GMP Grade C and D cleanrooms and regular environment sampling).


    Looking for the best air sampling?

    IUL’s Spin Air is a portable high-flow single-stage impaction-based air sampler for bacteria sampling that gives the highest concentration with the lowest data dispersion, being the most precise, accurate, and reproducible air sampling.

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