Eddy Jet 2W – Spiral plater
Eddy Jet 2W reduces costs per test, hands on time, and consumable use during counts while increasing microbiology lab’s productivity.
This cutting-edge spiral plater automates, standardizes and streamlines plate inoculations with its patented cross-contamination-free technology. Microsyringes give the system unique, unmatched advantages that make it a bestseller. The user interface is very intuitive and there are no setup times.
Although Eddy Jet 2W is mostly used by food, cosmetic and pharmaceutical labs, any microbiology lab can greatly benefit from its use. This instrument will generate a threefold dilution concentration gradient on a single plate, allowing labs to skip serial dilutions and most platings. Each sample is processed with a brand new sterile microsyringe, making sample handling a cross-contamination-free process.
Other than bacterial counts, antimicrobial susceptibility testing (AST) and mutagenicity test applications are also available.
System Highlights
Sterility: An exclusive patented microsyringe technology allows for real sampling sterility. Since no disinfectants are used, Eddy Jet 2W does away with any possible false-negative results due to disinfectant carryover.
Simplicity: Get trained in less than 5 minutes: insert a Petri plate, press on the “play button” on the Eddy Jet 2W innovative color touch screen, and that’s it— PLUG & PLATE!
Fully Validated: Spiral plating is a fully validated microbiological method, see: ISO 7218/ AOAC 977.27/ FDA (BAM Ch.3). Verification protocols are available for the device’s spirals.
Advantageous Mechanics: Eddy Jet’s spiral plater dependable Stepper Microcontroller motors regulate the instrument’s high precision microsyringe liquid dispensing. This approach prevents all the issues of competing technologies where cleaning cycles, disinfectant preparation, cross-contamination, unnecessary and complex liquid handling, stylus calibration, noisy vacuum pumps, and complicated maintenance requirements overshadow the advantages of spiral plating.
Connectivity: Eddy Jet 2W spiral plater features cutting-edge connectivity; it can be connected to a barcode scanner and a printer in order to provide plate traceability. Its USB and Ethernet connections allow for the connection of a keyboard for data entry or a pen drive for upgrades.
Application scope
Many samples can be processed in Eddy Jet 2W; aliquots taken from solutions of any diluted sample are placed inside a gamma-irradiated beaker, specially devised, and introduced inside Eddy Jet 2W for processing. During dispensation the device can perform up to 37 types of spirals. Eddy Jet 2W inoculates 90 and 150 mm plates alike thanks to its special plate holder device. Using 150 mm plates enables generating an even larger concentration gradient having a single plate account for almost 5 log10 concentration range.
Spiral plating is most frequently used for counting samples but several other applications exist. Thanks to the lawn spiral distribution, Eddy Jet 2W can be used to create bacterial lawns meant to be used in AST for Kirby-Bauer testing. Spiral gradient enables testing susceptibilities of multiple strains to a single antibiotic. The Spiral Salmonella assay for mutagenicity testing, a spiral plating equivalent of the AMES test, can also be performed using Eddy Jet 2W. Eddy Jet 2W linear modes enable refining the detection of mutagenic concentration thresholds.
How to continue processing diluted samples
Initially samples are suspended in a diluent broth through gravimetric dilutions performed in either the Smart Dilutor W or Pinch dilutor. Sample-containing bags can be stored in Portabag bagholders, pending homogenization in Masticator blenders. When homogenizing samples, filter bags are to be used for debris-generating samples while, for other samples, filterless bags suffice. The Eddy Jet 2 spiral plater is used to reduce the number of plates that are plated, generating a threefold decimal dilution counting range on a single plate. Following incubation, plates are automatically counted with the SphereFlash® or manually with the DOT®.
Ordering information
| Description | Cat.No. |
|---|---|
| Eddy Jet 2W | 90003800 |
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Technical Specifications
| Reproducibility | > 99% |
| Time for a full cycle | 30 s (disposable syringes make disinfection unnecessary) |
| Spreading modes stored | 25 spread modes (37 counting those for 150 mm Petri plates) |
| Petri plate diameters | 90 and 150 mm |
| Minimum step volume | < 10 nl |
| Maximum pipetted volume | 100 μl |
| Syringe material | Polypropylene |
| Plunger material | Polyethylene H.D. |
| Auto checking | Programmed for 6 regions |
| Touch screen | 7 in |
| External balance connection | Serial RS232 DB9 |
| Printer connection | Serial RS232 DB9 |
| Barcode scanner connection | Specific connection for IUL barcode scanner |
| Connection for updates | Ethernet/ USB pen drive |
| Additional connections for peripherals | 4 × USB Host ports |
| Dimensions (W×H×D) | 51.5 × 42 × 50 cm / 20.3 × 16.5 × 19.7 in |
| Weight | 20 kg / 44 lb. |
| Motion control | 3 Microcontroller-regulated stepper motors |
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ACCESSORIES AND CONSUMABLES
| Description | Cat. No. |
| 1000 gamma-irradiated Microsyringes | 90001780 |
| 1000 gamma-irradiated Sample Beakers | 90001790 |
| Plate for 15 cm Petri Dishes | 90001715 |
| Holder for sample Beaker box | 90001949 |
| Holder for Microsyringes box | 900011275 |
| Sampling holder for Beakers | 90001974 |
| Barcode Scanner | 90005701 |